Review



hexanediol aladdin  (MedChemExpress)


Bioz Verified Symbol MedChemExpress is a verified supplier
Bioz Manufacturer Symbol MedChemExpress manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    MedChemExpress hexanediol aladdin
    Figure 1. GATA3 undergoes PS in cells and in vitro (A) Confocal microscopy images of HEK293T cells expressing GATA3WT-mEGFP (green). Nuclei were stained with DAPI (purple). Scale bar, 5 μm. (B) Line-scan intensity profiles of GATA3WT-mEGFP and DAPI signals along the yellow arrow in (A). (C) Time-lapse snapshots of fluorescence recovery after photobleaching (FRAP) in HEK293T cells expressing GATA3WT-mEGFP. Scale bar, 2 μm. (D) FRAP recovery kinetics of GATA3WT-mEGFP condensates (n = 3). t1/2, half-recovery time. (E) Representative snapshots of HEK293T expressing GATA3WT-mEGFP <t>with</t> <t>5%</t> <t>1,6-hexanediol</t> treatment across the indicated time. Scale bar, 2 μm. (F) Representative images of 10 μM purified MBP-GATA3WT protein solution in tubes and under microscope with or without TEV protease treatment. Scale bar, 20 μm.
    Hexanediol Aladdin, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hexanediol aladdin/product/MedChemExpress
    Average 93 stars, based on 1 article reviews
    hexanediol aladdin - by Bioz Stars, 2026-03
    93/100 stars

    Images

    1) Product Images from "GATA3 differentially regulates the transcriptome via zinc finger 2-modulated phase separation."

    Article Title: GATA3 differentially regulates the transcriptome via zinc finger 2-modulated phase separation.

    Journal: Cell reports

    doi: 10.1016/j.celrep.2025.115702

    Figure 1. GATA3 undergoes PS in cells and in vitro (A) Confocal microscopy images of HEK293T cells expressing GATA3WT-mEGFP (green). Nuclei were stained with DAPI (purple). Scale bar, 5 μm. (B) Line-scan intensity profiles of GATA3WT-mEGFP and DAPI signals along the yellow arrow in (A). (C) Time-lapse snapshots of fluorescence recovery after photobleaching (FRAP) in HEK293T cells expressing GATA3WT-mEGFP. Scale bar, 2 μm. (D) FRAP recovery kinetics of GATA3WT-mEGFP condensates (n = 3). t1/2, half-recovery time. (E) Representative snapshots of HEK293T expressing GATA3WT-mEGFP with 5% 1,6-hexanediol treatment across the indicated time. Scale bar, 2 μm. (F) Representative images of 10 μM purified MBP-GATA3WT protein solution in tubes and under microscope with or without TEV protease treatment. Scale bar, 20 μm.
    Figure Legend Snippet: Figure 1. GATA3 undergoes PS in cells and in vitro (A) Confocal microscopy images of HEK293T cells expressing GATA3WT-mEGFP (green). Nuclei were stained with DAPI (purple). Scale bar, 5 μm. (B) Line-scan intensity profiles of GATA3WT-mEGFP and DAPI signals along the yellow arrow in (A). (C) Time-lapse snapshots of fluorescence recovery after photobleaching (FRAP) in HEK293T cells expressing GATA3WT-mEGFP. Scale bar, 2 μm. (D) FRAP recovery kinetics of GATA3WT-mEGFP condensates (n = 3). t1/2, half-recovery time. (E) Representative snapshots of HEK293T expressing GATA3WT-mEGFP with 5% 1,6-hexanediol treatment across the indicated time. Scale bar, 2 μm. (F) Representative images of 10 μM purified MBP-GATA3WT protein solution in tubes and under microscope with or without TEV protease treatment. Scale bar, 20 μm.

    Techniques Used: In Vitro, Confocal Microscopy, Expressing, Staining, Fluorescence, Purification, Microscopy

    Figure 3. Positively charged amino acids of ZnF2 modulate GATA3 condensate formation (A) Representative images of droplet formation of 5 μM GATA3WT-mEGFP in the presence of 10% 1,6-hexanediol (1,6-Hex). (B) Turbidity measurements of GATA3WT-mEGFP solutions with and without 10% 1,6-Hex treatment. Data are presented as means ±SD. ***p < 0.001. (C) Representative images of GATA3WT-mEGFP droplets at increasing NaCl concentrations ranging from 37.5 to 300 mM, as indicated. Scale bar, 5 μm. (D) Quantification of droplet area corresponding to (C). Data are presented as means ± SD. **p < 0.01 and ***p < 0.001. (E) Net charge distribution analysis of the GATA3 protein sequence. Red indicates negatively charged residues; blue indicates positively charged residues. The DBD region is highlighted in orange. Lysine (K) and arginine (R) residues within the DBD are specifically marked in blue font. (F) Representative images of HEK293T cells expressing GATA3WT-mEGFP and its mutants, as indicated. Scale bar, 5 μm. (G) Quantification of the number of condensates in HEK293T cells expressing GATA3WT-mEGFP and its mutants. Data are presented as means ± SD. *p < 0.05 and ***p < 0.001. (H) Representative snapshots from FRAP assays in HEK293T cells expressing GATA3WT-mEGFP or its mutants, as indicated. Scale bar, 2 μm. (I) Quantitative analysis of the average fluorescence recovery of GATA3WT-mEGFP condensates and their mutants (n = 3), as shown in (H).
    Figure Legend Snippet: Figure 3. Positively charged amino acids of ZnF2 modulate GATA3 condensate formation (A) Representative images of droplet formation of 5 μM GATA3WT-mEGFP in the presence of 10% 1,6-hexanediol (1,6-Hex). (B) Turbidity measurements of GATA3WT-mEGFP solutions with and without 10% 1,6-Hex treatment. Data are presented as means ±SD. ***p < 0.001. (C) Representative images of GATA3WT-mEGFP droplets at increasing NaCl concentrations ranging from 37.5 to 300 mM, as indicated. Scale bar, 5 μm. (D) Quantification of droplet area corresponding to (C). Data are presented as means ± SD. **p < 0.01 and ***p < 0.001. (E) Net charge distribution analysis of the GATA3 protein sequence. Red indicates negatively charged residues; blue indicates positively charged residues. The DBD region is highlighted in orange. Lysine (K) and arginine (R) residues within the DBD are specifically marked in blue font. (F) Representative images of HEK293T cells expressing GATA3WT-mEGFP and its mutants, as indicated. Scale bar, 5 μm. (G) Quantification of the number of condensates in HEK293T cells expressing GATA3WT-mEGFP and its mutants. Data are presented as means ± SD. *p < 0.05 and ***p < 0.001. (H) Representative snapshots from FRAP assays in HEK293T cells expressing GATA3WT-mEGFP or its mutants, as indicated. Scale bar, 2 μm. (I) Quantitative analysis of the average fluorescence recovery of GATA3WT-mEGFP condensates and their mutants (n = 3), as shown in (H).

    Techniques Used: Sequencing, Expressing, Fluorescence



    Similar Products

    hexanediol aladdin  (MedChemExpress)
    93
    MedChemExpress hexanediol aladdin
    Figure 1. GATA3 undergoes PS in cells and in vitro (A) Confocal microscopy images of HEK293T cells expressing GATA3WT-mEGFP (green). Nuclei were stained with DAPI (purple). Scale bar, 5 μm. (B) Line-scan intensity profiles of GATA3WT-mEGFP and DAPI signals along the yellow arrow in (A). (C) Time-lapse snapshots of fluorescence recovery after photobleaching (FRAP) in HEK293T cells expressing GATA3WT-mEGFP. Scale bar, 2 μm. (D) FRAP recovery kinetics of GATA3WT-mEGFP condensates (n = 3). t1/2, half-recovery time. (E) Representative snapshots of HEK293T expressing GATA3WT-mEGFP <t>with</t> <t>5%</t> <t>1,6-hexanediol</t> treatment across the indicated time. Scale bar, 2 μm. (F) Representative images of 10 μM purified MBP-GATA3WT protein solution in tubes and under microscope with or without TEV protease treatment. Scale bar, 20 μm.
    Hexanediol Aladdin, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hexanediol aladdin/product/MedChemExpress
    Average 93 stars, based on 1 article reviews
    hexanediol aladdin - by Bioz Stars, 2026-03
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 1. GATA3 undergoes PS in cells and in vitro (A) Confocal microscopy images of HEK293T cells expressing GATA3WT-mEGFP (green). Nuclei were stained with DAPI (purple). Scale bar, 5 μm. (B) Line-scan intensity profiles of GATA3WT-mEGFP and DAPI signals along the yellow arrow in (A). (C) Time-lapse snapshots of fluorescence recovery after photobleaching (FRAP) in HEK293T cells expressing GATA3WT-mEGFP. Scale bar, 2 μm. (D) FRAP recovery kinetics of GATA3WT-mEGFP condensates (n = 3). t1/2, half-recovery time. (E) Representative snapshots of HEK293T expressing GATA3WT-mEGFP with 5% 1,6-hexanediol treatment across the indicated time. Scale bar, 2 μm. (F) Representative images of 10 μM purified MBP-GATA3WT protein solution in tubes and under microscope with or without TEV protease treatment. Scale bar, 20 μm.

    Journal: Cell reports

    Article Title: GATA3 differentially regulates the transcriptome via zinc finger 2-modulated phase separation.

    doi: 10.1016/j.celrep.2025.115702

    Figure Lengend Snippet: Figure 1. GATA3 undergoes PS in cells and in vitro (A) Confocal microscopy images of HEK293T cells expressing GATA3WT-mEGFP (green). Nuclei were stained with DAPI (purple). Scale bar, 5 μm. (B) Line-scan intensity profiles of GATA3WT-mEGFP and DAPI signals along the yellow arrow in (A). (C) Time-lapse snapshots of fluorescence recovery after photobleaching (FRAP) in HEK293T cells expressing GATA3WT-mEGFP. Scale bar, 2 μm. (D) FRAP recovery kinetics of GATA3WT-mEGFP condensates (n = 3). t1/2, half-recovery time. (E) Representative snapshots of HEK293T expressing GATA3WT-mEGFP with 5% 1,6-hexanediol treatment across the indicated time. Scale bar, 2 μm. (F) Representative images of 10 μM purified MBP-GATA3WT protein solution in tubes and under microscope with or without TEV protease treatment. Scale bar, 20 μm.

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GATA3 abcam Cat# ab199428 GAPDH abcam Cat# ab8245 H3K27ac abcam Cat# ab4729 H3K9me3 abcam Cat# ab8898 H3 abcam Cat# ab1791 suv39H1 abcam Cat# ab283262 Anti-RNA polymerase II (phospho S2) abcam Cat# ab193468 Anti-KAT3B / p300 abcam Cat# ab275378 Rabbit polyclonal to GFP abcam Cat# ab290 Rig-I/DDX58 Rabbit mAb ABclonal Cat# A22478 IRF-3 Rabbit mAb Cell Signaling Technology Cat# 4302 Phospho-IRF-3 (Ser386) Rabbit mAb Cell Signaling Technology Cat# 37829 Phospho-IRF-3 (Ser396) Rabbit mAb Cell Signaling Technology Cat# 4947 HA-Tag Rabbit mAb ABclonal Cat# AE105 TBK1/NAK Rabbit mAb ABclonal Cat# A3458 Donkey anti-goat IgG-HRP abcam Cat# ab6885 Phospho-TBK1/NAK (Ser172) Rabbit mAb Cell Signaling Technology Cat# 5483 Anti-KMT1E/SETDB1 abcam Cat# ab300573 Estrogen Receptor alpha Santa Cruz Cat# sc-73562 PML Santa Cruz Cat# sc-71910 Anti-SC35 abcam Cat# ab204916 Mouse monoclonal [IH10] to Coilin abcam Cat# ab87913 Bacterial and virus strains E. coli DH5α Lab stored N/A E. coli BL21/DE3 Lab stored N/A Lentivirus Lab stored N/A Chemicals, peptides, and recombinant proteins IPTG Sigma-Aldrich Cat# I6758 PMSF Sigma-Aldrich Cat# 329-98-6 LipofectamineTM 2000 Thermo Fisher Scientific Cat# 11668027 Puromycin Sigma-Aldrich Cat# 540222 Chaetocin MCE Cat# 28097-03-2 5-Azacytidine MCE Cat# 320-67-2 β-Estradiol MCE Cat# 50-28-2 Cycloheximide MCE Cat# 66-81-9 1,6-Hexanediol aladdin Cat# 629-11-8 X-tremeGENETM HP DNA Sigma-Aldrich Cat# 6366244001 Recombinant MBP-GATA3-WT protein This study N/A Recombinant MBP-GATA3-N protein This study N/A Recombinant MBP-GATA3-C protein This study N/A Recombinant MBP-GATA3-DBD protein This study N/A Recombinant MBP-GATA3-ΔN protein This study N/A Recombinant MBP-GATA3-ΔC protein This study N/A (Continued on next page) 18 Cell Reports 44, 115702, May 27, 2025

    Techniques: In Vitro, Confocal Microscopy, Expressing, Staining, Fluorescence, Purification, Microscopy

    Figure 3. Positively charged amino acids of ZnF2 modulate GATA3 condensate formation (A) Representative images of droplet formation of 5 μM GATA3WT-mEGFP in the presence of 10% 1,6-hexanediol (1,6-Hex). (B) Turbidity measurements of GATA3WT-mEGFP solutions with and without 10% 1,6-Hex treatment. Data are presented as means ±SD. ***p < 0.001. (C) Representative images of GATA3WT-mEGFP droplets at increasing NaCl concentrations ranging from 37.5 to 300 mM, as indicated. Scale bar, 5 μm. (D) Quantification of droplet area corresponding to (C). Data are presented as means ± SD. **p < 0.01 and ***p < 0.001. (E) Net charge distribution analysis of the GATA3 protein sequence. Red indicates negatively charged residues; blue indicates positively charged residues. The DBD region is highlighted in orange. Lysine (K) and arginine (R) residues within the DBD are specifically marked in blue font. (F) Representative images of HEK293T cells expressing GATA3WT-mEGFP and its mutants, as indicated. Scale bar, 5 μm. (G) Quantification of the number of condensates in HEK293T cells expressing GATA3WT-mEGFP and its mutants. Data are presented as means ± SD. *p < 0.05 and ***p < 0.001. (H) Representative snapshots from FRAP assays in HEK293T cells expressing GATA3WT-mEGFP or its mutants, as indicated. Scale bar, 2 μm. (I) Quantitative analysis of the average fluorescence recovery of GATA3WT-mEGFP condensates and their mutants (n = 3), as shown in (H).

    Journal: Cell reports

    Article Title: GATA3 differentially regulates the transcriptome via zinc finger 2-modulated phase separation.

    doi: 10.1016/j.celrep.2025.115702

    Figure Lengend Snippet: Figure 3. Positively charged amino acids of ZnF2 modulate GATA3 condensate formation (A) Representative images of droplet formation of 5 μM GATA3WT-mEGFP in the presence of 10% 1,6-hexanediol (1,6-Hex). (B) Turbidity measurements of GATA3WT-mEGFP solutions with and without 10% 1,6-Hex treatment. Data are presented as means ±SD. ***p < 0.001. (C) Representative images of GATA3WT-mEGFP droplets at increasing NaCl concentrations ranging from 37.5 to 300 mM, as indicated. Scale bar, 5 μm. (D) Quantification of droplet area corresponding to (C). Data are presented as means ± SD. **p < 0.01 and ***p < 0.001. (E) Net charge distribution analysis of the GATA3 protein sequence. Red indicates negatively charged residues; blue indicates positively charged residues. The DBD region is highlighted in orange. Lysine (K) and arginine (R) residues within the DBD are specifically marked in blue font. (F) Representative images of HEK293T cells expressing GATA3WT-mEGFP and its mutants, as indicated. Scale bar, 5 μm. (G) Quantification of the number of condensates in HEK293T cells expressing GATA3WT-mEGFP and its mutants. Data are presented as means ± SD. *p < 0.05 and ***p < 0.001. (H) Representative snapshots from FRAP assays in HEK293T cells expressing GATA3WT-mEGFP or its mutants, as indicated. Scale bar, 2 μm. (I) Quantitative analysis of the average fluorescence recovery of GATA3WT-mEGFP condensates and their mutants (n = 3), as shown in (H).

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GATA3 abcam Cat# ab199428 GAPDH abcam Cat# ab8245 H3K27ac abcam Cat# ab4729 H3K9me3 abcam Cat# ab8898 H3 abcam Cat# ab1791 suv39H1 abcam Cat# ab283262 Anti-RNA polymerase II (phospho S2) abcam Cat# ab193468 Anti-KAT3B / p300 abcam Cat# ab275378 Rabbit polyclonal to GFP abcam Cat# ab290 Rig-I/DDX58 Rabbit mAb ABclonal Cat# A22478 IRF-3 Rabbit mAb Cell Signaling Technology Cat# 4302 Phospho-IRF-3 (Ser386) Rabbit mAb Cell Signaling Technology Cat# 37829 Phospho-IRF-3 (Ser396) Rabbit mAb Cell Signaling Technology Cat# 4947 HA-Tag Rabbit mAb ABclonal Cat# AE105 TBK1/NAK Rabbit mAb ABclonal Cat# A3458 Donkey anti-goat IgG-HRP abcam Cat# ab6885 Phospho-TBK1/NAK (Ser172) Rabbit mAb Cell Signaling Technology Cat# 5483 Anti-KMT1E/SETDB1 abcam Cat# ab300573 Estrogen Receptor alpha Santa Cruz Cat# sc-73562 PML Santa Cruz Cat# sc-71910 Anti-SC35 abcam Cat# ab204916 Mouse monoclonal [IH10] to Coilin abcam Cat# ab87913 Bacterial and virus strains E. coli DH5α Lab stored N/A E. coli BL21/DE3 Lab stored N/A Lentivirus Lab stored N/A Chemicals, peptides, and recombinant proteins IPTG Sigma-Aldrich Cat# I6758 PMSF Sigma-Aldrich Cat# 329-98-6 LipofectamineTM 2000 Thermo Fisher Scientific Cat# 11668027 Puromycin Sigma-Aldrich Cat# 540222 Chaetocin MCE Cat# 28097-03-2 5-Azacytidine MCE Cat# 320-67-2 β-Estradiol MCE Cat# 50-28-2 Cycloheximide MCE Cat# 66-81-9 1,6-Hexanediol aladdin Cat# 629-11-8 X-tremeGENETM HP DNA Sigma-Aldrich Cat# 6366244001 Recombinant MBP-GATA3-WT protein This study N/A Recombinant MBP-GATA3-N protein This study N/A Recombinant MBP-GATA3-C protein This study N/A Recombinant MBP-GATA3-DBD protein This study N/A Recombinant MBP-GATA3-ΔN protein This study N/A Recombinant MBP-GATA3-ΔC protein This study N/A (Continued on next page) 18 Cell Reports 44, 115702, May 27, 2025

    Techniques: Sequencing, Expressing, Fluorescence